• β-mannase
• Acidic protease
• Pectinase
• Xylanase
• β-glucanase

Acidic protease

I. Physical appearance and technical specifications

Spec	Solid dosage form			Liquid dosage form
Physical appearance	Industrial grade	Feed grade	Food grade	Food grade
	Yellow brown powder	Yellow brown	Milky yellow powder	Brown liquid
Enzyme activity unit u/g or u/ml	40000	20000	20000	20000
	50000	25000	25000
	40000-80000	30000-80000	20000-30000
Working temperature	30-45℃
Working pH	2.5-5.0
Fineness or density	Standard sieve 40 mesh	Standard sieve 40 mesh	Standard sieve 50 mesh	＜1.2g/ml
	≥80%	60%	98%
Product standard	QB1805.3

Enzyme activity unit definition: 1g enzyme powder or 1ml enzyme liquid hydrolyze casein and produce 1μg tyrosine in 1 minute under the condition of 40 ℃, pH 3.0. Unit is u/g or u/ml.

II. Application

Acidic protease is a high-tech product produced by modern bioengineering technology. It is the enzyme to degrade protein. For the working pH of it is 2.5-5.0, it is called acidic protease.
1. Industrial grade acidic protease is mainly used in the industries that utilize corn, rice, wheat and other high starch content to produce alcohol and wine. Use in alcoholic fermentation can increase liquor rate of 0.3-0.5, can shorten fermentation period and reduce energy consumption.
2. Food grade acidic protease is used as food additive. As starch modifier, used in the production of bread, cake and sausage to improve the flavor and quality. Used in soy sauce production to increase amino acid content and used in beer to replace papain and bromelain.
3. When feed grade acidic protease and the feed consumed by animals, it will hydrolyze protein to peptide and amino acid to promote digestion and absorption and increase feed utilization and feed conversion.
4. Acidic protease has a good performance on fur softening. It can soften and fasten the feather and increase the luster for better dyeing to improve the quality and outturn percentage.

III. Using methods

1. Dosage
         In alcohol fermentation from corn, the dosage is between 10-16 units per gram of ingredient, depending on ingredients and specific process. Generally, the dosage is 14 units per gram ingredient, means 50000 units per ton ingredient, the dosage is 280g. If 40000 units, the dosage is 350g. The dosage depend on customers' need and specific situation in feed and food industry. Reference dosage 5-10u/g. 25u/g in soy sauce production. 5u/g in beer production.
2. Methods
A. Alcohol batch fermentation: After filling 1/3 of the ingredients into the fermenting cellar, add a full amount of activated food grade acidic protease. The dosage is determined by the ingredients quantity in the cellar.
B. Alcohol continuous fermentation: Add in the mash liquid discharge port of fermentor. The flow speed is according inlet amount.
C. Solid fermentation to produce alcohol: After cooling down the steamed fermented grains, add the whole required amount of acidic protease and mix evenly.
D. Solid fermentation to produce saccharomycetes or single cell protein feed: Cool the steamed ingredients to 30℃. Add 20units of acidic proteaser per gram ingredient to lukewarm water. After dissolved, add it to the ingredients, mix evenly and start fermenting.

IV. Precautions
1. When using acidic protease, dissolve it in 35-40℃ water at a 1:20 ratio for 1 hour with stirring every 15 minutes.
2. When using acidic protease, the yeast growth becomes rapid and the temperature will raise dramatically. So the temperature must be controlled under 40℃ or the using effect will be influenced.
3. In food and fee industry, the dosage and using methods should be determined based on specific factors.

V. Packing, storage and transportation
Solid dosage form: Plastic bag, net weight 2kg (small) or 20kg (large).
Liquid dosage form: Plastic bucket, net weight 25kg/bucket.
Stored in a cool and dry place and prevent from rain, moisture and direct sunlight.
Solid, if stored at 15℃, the enzyme activity unit of it will be no less than standard value for 3 months.
Liquid, if stored in low temperature (在5-10℃), the enzyme activity unit of it will be no less than standard value for 3 months.